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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so could add to tumorigenesis. This protein was discovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two supplemental splice variants have been noted but in-depth nucleotide sequence info just isn't however out there.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to make sure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity website is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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